CRISPR

Neville Sanjana explored noncoding genomic regions by combining pooled CRISPR screening and single cell sequencing.

An immune-inhibiting protein that regulates autophagy halts breast cancers from venturing across tissue borders.

A new prime editing sensor library targeting p53 mutations allowed scientists to interrogate thousands of tumor genotypes as they arose in endogenous contexts.

Base editors and prime editors help researchers perform more precise in vivo and ex vivo translational research.

Learn how cutting-edge sequencing techniques accelerate basic and disease research.

Multi-targeted Cas13 screens help researchers avoid permanent DNA cuts and evade T cell exhaustion.

Scientists hoping to treat immunodeficiencies using gene therapy have found a way to edit stem cells in mice without disrupting gene regulation.

Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.

Researchers are using CRISPR for precise genetic manipulation of human-associated microbes as a promising avenue for improving human health.

Genetic engineering pioneer Kevin Esvelt’s work highlights biotechnology’s immense potential for good—but also for catastrophe.

Jonathan Weissman and Luke Gilbert share how they developed several CRISPR-based epigenetic editors and how these tools differ from traditional CRISPR.

Shondra Pruett-Miller has taken many paths in her career with her love of genome editing always as a guiding light.

Understanding how Cas9 binds off-target sequences can help researchers refine CRISPR-mediated genome editing.

A 3D variation of pooled CRISPR screens could connect the dots between autism spectrum disorder genetics and cell fate pathways in the developing brain.

CRISPR plasmids provide a robust solution for streamlining human cell transfection workflows.   

A risk-benefit analysis of gene editing tools in stem cells revealed that base and prime editing carry vulnerabilities similar to those of CRISPR-Cas9, but at a reduced rate. 

Natural killer (NK) cells target infected and oncogenic cells, but are difficult to work with in vitro. Discover novel approaches to producing genetically modified NK cells for cell therapy. 

Recombinase polymerase amplification lets researchers rapidly replicate DNA in the clinic, in the field, or even in the International Space Station.

Samantha Maragh has taken on the difficult challenge of standardizing assays, data norms, and terminology in the ever evolving genome editing field.

Researchers create and improve CRISPR-Cas mediated gene editing technologies based on prokaryotic CRISPR systems and eukaryotic DNA repair mechanisms.