Home
Subjects
fluorescence

fluorescence

A cell with gold particles inside is hit with a red laser.

How Flow Cytometry Spurred Cell Biology

Shelby Bradford, PhD | Jun 14, 2024 | 10+ min read

The ability to rapidly count and measure features of cells opened the research flood gates.

Discover How Automated Microscopy Streamlines Multiplexed Apoptosis Assays

Multiplexing Caspase-Based Apoptosis Assays with Automated Microscopy

Leica Microsystems | May 29, 2024 | 1 min read

A state-of-the-art automated microscope streamlines data acquisition and analysis for multiplex assays.

A scientist loads a 96-well plate containing qPCR reactions into a thermal cycler for DNA amplification and detection.

Insights into qPCR: Protocol, Detection Methods, and Analysis

Tanuka Biswas, PhD | Nov 8, 2023 | 6 min read

Learn about quantitative PCR (qPCR), including its fundamentals, common applications, modes of detection, and key quantification methods.

Discover How CF® Dyes Let Scientists Delve Deeper into Biological Phenomena

CF^® Dyes: Clearer Fluorescent Results

Biotium | Sep 8, 2023 | 1 min read

CF^® Dyes combine pegylation and sulfonation to create fluorescent dyes with better signal intensity, more solubility, and superior specificity.

A multicolored illustration of a cell undergoing division.

See Beyond the Scatter Plot with Imaging, Spectral Flow Cytometry

The Scientist and BD Biosciences | Aug 9, 2023 | 3 min read

A novel instrument combines fluorescence-activated cell sorting, imaging flow cytometry, and spectral flow cytometry to advance cell population examination.

Lipofuscin autofluorescence was quenched using EverBrite TrueBlack® Hardset Mounting Medium®, allowing effective visualization of glial cells (GFAP antibody stain, cyan) and cell nuclei (magenta) in human cerebral cortex cryosections.

Stepping Out of the Background

The Scientist and Biotium | Jul 27, 2023 | 4 min read

New developments in non-specific fluorescence quenching offer researchers clearer pictures and more multiplexing possibilities.

Illustration of newly discovered mechanism allowing kinesin to “walk” down a microtubule. A green kinesin molecule with an attached yellow fluorophore is shown passing through a blue laser as it rotates step by step along a red and purple microtubule, fueled by blue ATP molecules that are hydrolyzed into orange ADP and phosphate groups.

High-Resolution Microscope Watches Proteins Strut Their Stuff

Holly Barker, PhD | Mar 31, 2023 | 3 min read

Modification on a high-resolution fluorescent microscopy technique allow researchers to track the precise movements of motor proteins.

Artist’s rendition of translucent teal proteins comprised of red spherical amino acids

Now AI Can Be Used to Design New Proteins

Kamal Nahas, PhD | Mar 3, 2023 | 4 min read

Machine learning can be harnessed to synthesize artificial light-bearing enzymes that actually work in cells.

Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.

Choosing Fluorescent Reagents for Every Live Cell Application

The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read

Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.

Learn About the Essential Principles and Practices of Flow Cytometry

Best Practices for Designing and Executing Successful Flow Cytometry Experiments

Fortis Life Sciences | Sep 6, 2022 | 1 min read

A roadmap to navigating flow cytometry

Modernizing Drug Discovery with Flow Cytometry

Bio-Rad | Aug 23, 2022 | 1 min read

Automation, high-throughput capabilities, and improved technologies and reagents accelerate cell profiling and screening.

To flag neurons that have experienced genotoxic stress, researchers developed an in vivo sensor using an adeno-associated viral vector, called PRISM. Because a cell’s DNA damage response (DDR)—which activates in response to stressors such as environmental toxins or the buildup of misfolded proteins—also responds to invading pathogens, PRISM has an easier time transfecting cells whose damage response mechanisms are preoccupied with existing DNA damage. Once inside, the virus hijacks the neuron’s DNA replication machinery, which reverts an engineered frameshift mutation in the virus and thereby prompts the production of a fluorescent protein that can be observed via microscopy.

Infographic: DNA Damage Viewed with Unprecedented Clarity

Amanda Heidt | Aug 15, 2022 | 2 min read

A new genetic sensor called PRISM makes use of a host cell’s DNA replication machinery to trigger fluorescence in neurons with damaged DNA.

DNA Damage Viewed with Unprecedented Clarity

Amanda Heidt | Aug 15, 2022 | 3 min read

A new tool called PRISM draws on virus-host interactions and a DNA repair pathway to help researchers visualize how cellular stress may contribute to neurodegenerative disease.